Human domain antibodies against virulence traits of Candida albicans inhibit fungus adherence to vaginal epithelium and protect against experimental vaginal candidiasis.
Article
De Bernardis, F., Liu, H., O'Mahony, R., La Valle, R., Bartollino, S., Sandini, S., Grant, S., Brewis, N., Tomlinson, I., Basset, R., Holton, J., Roitt, I. and Cassone, A. 2007. Human domain antibodies against virulence traits of Candida albicans inhibit fungus adherence to vaginal epithelium and protect against experimental vaginal candidiasis. Journal of Infectious Diseases. 195 (1), pp. 149-157. https://doi.org/10.1086/509891
Type | Article |
---|---|
Title | Human domain antibodies against virulence traits of Candida albicans inhibit fungus adherence to vaginal epithelium and protect against experimental vaginal candidiasis. |
Authors | De Bernardis, F., Liu, H., O'Mahony, R., La Valle, R., Bartollino, S., Sandini, S., Grant, S., Brewis, N., Tomlinson, I., Basset, R., Holton, J., Roitt, I. and Cassone, A. |
Abstract | Antibody variable domains (domain antibodies [DAbs]) are genetically engineered antibody fragments that include individual heavy‐chain (VH) or κ‐chain (Vκ) variable domains and lack the Fc region. Human DAbs against the 65‐kDa mannoprotein (MP65) or the secretory aspartyl proteinase (SAP)–2 of Candida albicans (monospecific DAbs) or against both fungal antigens (heterodimeric, bispecific DAbs) were generated from phage expression libraries. Both monospecific and bispecific DAbs inhibited fungus adherence to the epithelial cells of rat vagina and accelerated the clearance of vaginal infection with the fungus. When heterodimeric DAbs were used, the clearance of infection was at least equivalent to treatment with fluconazole. The in vivo protective effects of DAbs were demonstrated by both pre‐ and postchallenge schedules of DAb administration and with both fluconazole‐susceptible and fluconazole‐resistant strains of C. albicans. This is the first demonstration that human DAbs lacking the Fc constituent can efficiently control an infection and can act largely by inhibiting adherence. |
Research Group | Biomarkers for Cancer group |
Publisher | Infectious Diseases Society of America |
Journal | Journal of Infectious Diseases |
ISSN | 1537-6613 |
Publication dates | |
01 Jul 2007 | |
Publication process dates | |
Deposited | 11 Feb 2010 |
Output status | Published |
Digital Object Identifier (DOI) | https://doi.org/10.1086/509891 |
Language | English |
https://repository.mdx.ac.uk/item/82376
23
total views0
total downloads2
views this month0
downloads this month