Technical note: effects of arsenate (AS5+) on growth and production of glutathione (GSH) and phytochelatins (PCS) in Chlorella Vulgaris
Article
Jiang, Y., Purchase, D., Jones, H. and Garelick, H. 2011. Technical note: effects of arsenate (AS5+) on growth and production of glutathione (GSH) and phytochelatins (PCS) in Chlorella Vulgaris. International Journal of Phytoremediation. 13 (8), pp. 834-844. https://doi.org/10.1080/15226514.2010.525560
Type | Article |
---|---|
Title | Technical note: effects of arsenate (AS5+) on growth and production of glutathione (GSH) and phytochelatins (PCS) in Chlorella Vulgaris |
Authors | Jiang, Y., Purchase, D., Jones, H. and Garelick, H. |
Abstract | The effect of arsenate (As5+) on growth and chlorophyll a production in Chlorella vulgaris, its removal by C. vulgaris and the role of glutathione (GSH) and phytochelatins (PCs) were investigated. C. vulgaris was tolerant to As5+ at up to 200 mg/L and was capable of consistently removing around 70% of the As5+ present in growth media over a wide range of exposure concentrations. Spectral analysis revealed that PCs and their arsenic-combined complexes were absent indicating that the high bioaccumulation and tolerance to arsenic observed was not due to intracellular chelation. In contrast, GSH was found in all samples ranging from 0.8 mg/L in the control to 6.5mg/L in media containing 200 mg/L As5+ suggesting that GSH plays a more prominent role in the detoxification of As5+ in C. vulgaris than PC. At concentrations below 100 mg/L cell surface binding and other mechanisms may play the primary role in As5+ detoxification, whereas above this concentration As5+ begins to accumulate inside the algal cells and activates a number of intracellular cell defence mechanisms, such as increased production of GSH. The overall findings complement field studies which suggest C.vulgaris as an increasingly promising low cost As phytoremediation method for developing countries. |
Keywords | Chlorella vulgaris; arsenate accumulation; glutathione; phytochelatins |
Publisher | Taylor and Francis |
Journal | International Journal of Phytoremediation |
ISSN | 1522-6514 |
Electronic | 1549-7879 |
Publication dates | |
Online | 02 Jun 2011 |
Sep 2011 | |
Publication process dates | |
Deposited | 16 Mar 2012 |
Output status | Published |
Accepted author manuscript | |
Copyright Statement | This is an Accepted Manuscript of an article published by Taylor & Francis in International Journal of Phytoremediation on 02 Jun 2011, available at: https://doi.org/10.1080/15226514.2010.525560 |
Digital Object Identifier (DOI) | https://doi.org/10.1080/15226514.2010.525560 |
PubMed ID | 21972522 |
Web of Science identifier | WOS:000291268400008 |
Language | English |
https://repository.mdx.ac.uk/item/838xy
Download files
68
total views10
total downloads2
views this month0
downloads this month