Bacillus subtilis fadB (ysiB) gene encodes an enoyl-CoA hydratase

Article


Frandi, A., Zucca, P., Marvasi, M., Mastromei, G., Sanjust, E. and Perito, B. 2011. Bacillus subtilis fadB (ysiB) gene encodes an enoyl-CoA hydratase. Annals of Microbiology. 61 (2, Jou), pp. 371-374. https://doi.org/10.1007/s13213-010-0121-5
TypeArticle
TitleBacillus subtilis fadB (ysiB) gene encodes an enoyl-CoA hydratase
AuthorsFrandi, A., Zucca, P., Marvasi, M., Mastromei, G., Sanjust, E. and Perito, B.
Abstract

Fatty acids are essential components of membranes and are an important source of metabolic energy. In bacteria, the β-oxidation pathway is well known in Escherichia coli. Bacillus subtilis possesses a considerable number of genes, organized in five operons, that are most likely involved in the β-oxidation of fatty acids. Among these genes, only one product, FadRBs (YsiA), has been recently characterized as a transcriptional regulatory protein which negatively regulates the expression of β-oxidation genes including those belong- ing to the lcfA operon, including fadRBs (ysiA). The probable involvement of the FadRBs (YsiA) regulon members in β- oxidation is inferred from data based on BLASTP similarity of their gene products. In this work, we report the cloning and the expression of B. subtilis fadBBs(ysiB), belonging to the lcfA operon, and the functional characterization of its product as an enoyl-CoA hydratase, demonstrating the actual involvement of these genes in fatty acid β-oxidation.

Keywords�²-oxidation, Bacillus subtilis, fadB Bs, enoyl-CoA hydratase, lcfA operon
PublisherSpringer Verlag
JournalAnnals of Microbiology
ISSN1590-4261
Publication dates
Print19 Jan 2011
Publication process dates
Deposited06 May 2015
Accepted01 Jan 2011
Output statusPublished
Publisher's version
Additional information

J2: Ann Microbiol

Digital Object Identifier (DOI)https://doi.org/10.1007/s13213-010-0121-5
LanguageEnglish
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