Mass spectrometry imaging of 3D tissue models

Article


Russo, C., Lewis, E., Flint, L. and Clench, M. 2018. Mass spectrometry imaging of 3D tissue models. Proteomics. 18 (14). https://doi.org/10.1002/pmic.201700462
TypeArticle
TitleMass spectrometry imaging of 3D tissue models
AuthorsRusso, C., Lewis, E., Flint, L. and Clench, M.
Abstract

A 3D cell culture is an artificially created environment in which cells are permitted to grow/interact with their surroundings in all three dimensions. Derived from 3D cell culture, organoids are generally small-scale constructs of cells that are fabricated in the laboratory to serve as 3D representations of in vivo tissues and organs. Due to regulatory, economic and societal issues concerning the use of animals in scientific research, it seems clear that the use of 3D cell culture and organoids in for example early stage studies of drug efficacy and toxicity will increase. The combination of such 3D tissue models with mass spectrometry imaging provides a label-free methodology for the study of drug absorption/penetration, drug efficacy/toxicity, and drug biotransformation. In this article, some of the successes achieved to date and challenges to be overcome before this methodology is more widely adopted are discussed.

Keywordsliving skin equivalent models; skin; spheroids; toxicology
Sustainable Development Goals3 Good health and well-being
Middlesex University ThemeHealth & Wellbeing
LanguageEnglish
PublisherWiley
JournalProteomics
ISSN1615-9853
Electronic1615-9861
Publication dates
Online28 May 2018
Print31 Jul 2018
Publication process dates
Deposited19 Jun 2023
Submitted16 Mar 2018
Accepted20 Apr 2018
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1002/pmic.201700462
Web of Science identifierWOS:000440165100002
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