Quantitative investigation of terbinafine hydrochloride absorption into a living skin equivalent model by MALDI-MSI

Article


Russo, C., Brickelbank, N., Duckett, C., Mellor, S., Rumbelow, S. and Clench, M. 2018. Quantitative investigation of terbinafine hydrochloride absorption into a living skin equivalent model by MALDI-MSI. Analytical Chemistry. 90 (16), pp. 10031-10038. https://doi.org/10.1021/acs.analchem.8b02648
TypeArticle
TitleQuantitative investigation of terbinafine hydrochloride absorption into a living skin equivalent model by MALDI-MSI
AuthorsRusso, C., Brickelbank, N., Duckett, C., Mellor, S., Rumbelow, S. and Clench, M.
Abstract

The combination of microspotting of analytical and internal standards, matrix sublimation, and recently developed software for quantitative mass spectrometry imaging has been used to develop a high-resolution method for the determination of terbinafine hydrochloride in the epidermal region of a full thickness living skin equivalent model. A quantitative assessment of the effect of the addition of the penetration enhancer (dimethyl isosorbide (DMI)) to the delivery vehicle has also been performed, and data have been compared to those obtained from LC−MS/MS measurements of homogenates of isolated epidermal tissue. At 10% DMI, the levels of signal detected for the drug in the epidermis were 0.20 ± 0.072 mg/g tissue for QMSI and 0.28 ± 0.040 mg/g tissue for LC−MS/MS at 50% DMI 0.69 ± 0.23 mg/g tissue for QMSI and 0.66 ± 0.057 mg/g tissue for LC−MS/ MS. Comparison of means and standard deviations indicates no significant difference between the values obtained by the two methods.

Sustainable Development Goals3 Good health and well-being
Middlesex University ThemeHealth & Wellbeing
LanguageEnglish
PublisherAmerican Chemical Society
JournalAnalytical Chemistry
ISSN0003-2700
Electronic1520-6882
Publication dates
Online19 Jul 2018
Print21 Aug 2018
Publication process dates
Deposited19 Jun 2023
Accepted19 Jul 2018
Submitted12 Jun 2018
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1021/acs.analchem.8b02648
https://doi.org/10.1021/acs.analchem.8b02648#notes1
Web of Science identifierWOS:000442707100051
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