Spatially resolved quantitation of drug in skin equivalents using mass spectrometry imaging (MSI)

Book chapter

Russo, C. and Clench, M.R. 2023. Spatially resolved quantitation of drug in skin equivalents using mass spectrometry imaging (MSI). in: Cole, L. and Clench, M. (ed.) Imaging Mass Spectrometry: Methods and Protocols New York Springer. pp. 27-40
Chapter titleSpatially resolved quantitation of drug in skin equivalents using mass spectrometry imaging (MSI)
AuthorsRusso, C. and Clench, M.R.
Abstract

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) has seen a growing interest as a leading technique in the pharmaceutical industry for mapping label-free exogenous and endogenous species in biological tissues. However, the use of MALDI-MSI to perform spatially resolved absolute quantitation of species directly in tissues is still challenging, and robust quantitative mass spectrometry imaging (QMSI) methods need to be developed. In this study, we describe the microspotting technique for analytical and internal standard deposition, matrix sublimation, powerful QMSI software, and mass spectrometry imaging setup to obtain absolute quantitation of drug distribution in 3D skin models.

Keywords3D skin models; Internal standard; MALDI-MSI; Microspotting; QMSI; Quantitation
Sustainable Development Goals3 Good health and well-being
Middlesex University ThemeHealth & Wellbeing
Page range27-40
Book titleImaging Mass Spectrometry: Methods and Protocols
EditorsCole, L. and Clench, M.
PublisherSpringer
Place of publicationNew York
Edition2nd
SeriesMethods in Molecular Biology
ISBN
Hardcover9781071633182
Electronic9781071633199
Paperback9781071633212
ISSN1064-3745
Electronic1940-6029
Publication dates
Online07 Jul 2023
Print2023
Publication process dates
Accepted01 Jun 2023
Deposited05 Jun 2025
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1007/978-1-0716-3319-9_3
Related Output
Is part ofhttps://doi.org/10.1007/978-1-0716-3319-9
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