Mesenchymal stem cells with increased stromal cell-derived factor 1 expression enhanced fracture healing

Article


Ho, C., Sanghani, A., Hua, J., Coathup, M., Kalia, P. and Blunn, G. 2015. Mesenchymal stem cells with increased stromal cell-derived factor 1 expression enhanced fracture healing. Tissue Engineering Part A. 21 (3-4), pp. 594-602. https://doi.org/10.1089/ten.tea.2013.0762
TypeArticle
TitleMesenchymal stem cells with increased stromal cell-derived factor 1 expression enhanced fracture healing
AuthorsHo, C., Sanghani, A., Hua, J., Coathup, M., Kalia, P. and Blunn, G.
Abstract

Treatment of critical size bone defects pose a challenge in orthopedics. Stem cell therapy together with cytokines has the potential to improve bone repair as they cause the migration and homing of stem cells to the defect site. However, the engraftment, participation, and recruitment of other cells within the regenerating tissue are important. To enhance stem cell involvement, this study investigated overexpression of stem cells with stromal cell-derived factor 1 (SDF-1) using an adenovirus. We hypothesized that these engineered cells would effectively increase the migration of native cells to the site of fracture, enhancing bone repair. Before implantation, we showed that SDF-1 secreted by transfected cells increased the migration of nontransfected cells. In a rat defect bone model, bone marrow mesenchymal stem cells overexpressing SDF-1 showed significantly (p=0.003) more new bone formation within the gap and less bone mineral loss at the area adjacent to the defect site during the early bone healing stage. In conclusion, SDF-1 was shown to play an important role in accelerating fracture repair and contributing to bone repair in rat models, by recruiting more host stem cells to the defect site and encouraging osteogenic differentiation and production of bone.

PublisherMary Ann Liebert
JournalTissue Engineering Part A
ISSN1937-3368
Publication dates
Online06 Nov 2014
Print28 Feb 2015
Publication process dates
Deposited28 May 2015
Accepted12 Sep 2014
Output statusPublished
Publisher's version
Copyright Statement

Copyright 2015, Mary Ann Liebert, Inc.
This work is licensed under a Creative Commons Attribution 3.0 United States License. You are free to copy, distribute, transmit and adapt this work, but you must attribute this work as ‘‘Tissue Engineering, Part A. Copyright 2014 Mary Ann Liebert, Inc. http://liebertpub.com/tea, used under a Creative Commons Attribution License: http://creativecommons.org/licenses/by/3.0/us/’’

Digital Object Identifier (DOI)https://doi.org/10.1089/ten.tea.2013.0762
LanguageEnglish
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