Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces

Article


García-Gareta, E., Hua, J., Knowles, J. and Blunn, G. 2013. Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces. Journal of Materials Science: Materials in Medicine. 24 (1), pp. 199-210. https://doi.org/10.1007/s10856-012-4789-x
TypeArticle
TitleComparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces
AuthorsGarcía-Gareta, E., Hua, J., Knowles, J. and Blunn, G.
Abstract

The hypothesis for this study was that there is no difference in mesenchymal stem cells (MSCs) proliferation and osteogenic differentiation between calcium-phosphate (CaP) coatings with different crystal size deposited on different topographic surfaces of metal discs. Polished (P) and sand-blasted (SB) tantalum and TiAl6V4 discs were CaP coated by three methods-biomimetic (BioM), electrochemical at 20 mA/cm(2) and at 6.5 mA/cm(2)-and cultured with MSCs. At days 4, 7 and 14, cell proliferation-alamarBlue(®) activity and DNA quantification-and differentiation down the osteogenic lineage-ALP activity normalised per amount of DNA and SEM (morphology)-were analysed. Results showed that MSCs proliferated more when cultured on the nano-sized BioM coatings compared to uncoated and electrochemically coated discs. MSCs also proliferated more on P surfaces than on SB and or electrochemical coatings. All the coatings induced osteogenic differentiation, which was greater on electrochemical coatings and SB discs.

PublisherSpringer US
JournalJournal of Materials Science: Materials in Medicine
ISSN0957-4530
Publication dates
Online10 Oct 2012
Print01 Jan 2013
Publication process dates
Deposited28 May 2015
Accepted03 Oct 2012
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1007/s10856-012-4789-x
LanguageEnglish
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