Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces
Article
García-Gareta, E., Hua, J., Knowles, J. and Blunn, G. 2013. Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces. Journal of Materials Science: Materials in Medicine. 24 (1), pp. 199-210. https://doi.org/10.1007/s10856-012-4789-x
Type | Article |
---|---|
Title | Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces |
Authors | García-Gareta, E., Hua, J., Knowles, J. and Blunn, G. |
Abstract | The hypothesis for this study was that there is no difference in mesenchymal stem cells (MSCs) proliferation and osteogenic differentiation between calcium-phosphate (CaP) coatings with different crystal size deposited on different topographic surfaces of metal discs. Polished (P) and sand-blasted (SB) tantalum and TiAl6V4 discs were CaP coated by three methods-biomimetic (BioM), electrochemical at 20 mA/cm(2) and at 6.5 mA/cm(2)-and cultured with MSCs. At days 4, 7 and 14, cell proliferation-alamarBlue(®) activity and DNA quantification-and differentiation down the osteogenic lineage-ALP activity normalised per amount of DNA and SEM (morphology)-were analysed. Results showed that MSCs proliferated more when cultured on the nano-sized BioM coatings compared to uncoated and electrochemically coated discs. MSCs also proliferated more on P surfaces than on SB and or electrochemical coatings. All the coatings induced osteogenic differentiation, which was greater on electrochemical coatings and SB discs. |
Publisher | Springer US |
Journal | Journal of Materials Science: Materials in Medicine |
ISSN | 0957-4530 |
Publication dates | |
Online | 10 Oct 2012 |
01 Jan 2013 | |
Publication process dates | |
Deposited | 28 May 2015 |
Accepted | 03 Oct 2012 |
Output status | Published |
Digital Object Identifier (DOI) | https://doi.org/10.1007/s10856-012-4789-x |
Language | English |
https://repository.mdx.ac.uk/item/8571z
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