Screen key genes associated with distraction-induced osteogenesis of stem cells using bioinformatics methods

Article

Chen, J., Hua, J. and Song, W. 2021. Screen key genes associated with distraction-induced osteogenesis of stem cells using bioinformatics methods. International Journal of Molecular Sciences. 22 (12). https://doi.org/10.3390/ijms22126505
TypeArticle
TitleScreen key genes associated with distraction-induced osteogenesis of stem cells using bioinformatics methods
AuthorsChen, J., Hua, J. and Song, W.
Abstract

Background: Applying mesenchymal stem cells (MSCs), together with the distraction osteogenesis (DO) process, displayed enhanced bone quality and shorter treatment periods. The DO guides the differentiation of MSCs by providing mechanical clues. However, the underlying key genes and pathways are largely unknown. The aim of this study was to screen and identify hub genes involved in distraction-induced osteogenesis of MSCs and potential molecular mechanisms.
Material and Methods: The datasets were downloaded from the ArrayExpress database. Three samples of negative control and two samples subjected to 5% cyclic sinusoidal distraction at 0.25 Hz for 6 h were selected for screening differentially expressed genes (DEGs) and then analysed via bioinformatics methods. The Gene Ontology (GO) terms and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment were investigated. The protein–protein interaction (PPI) network was visualised through the Cytoscape software. Gene set enrichment analysis (GSEA) was conducted to verify the enrichment of a self-defined osteogenic gene sets collection and identify osteogenic hub genes.
Results: Three hub genes (IL6, MMP2, and EP300) that were highly associated with distraction-induced osteogenesis of MSCs were identified via the Venn diagram. These hub genes could provide a new understanding of distraction-induced osteogenic differentiation of MSCs and serve as potential gene targets for optimising DO via targeted therapies.

KeywordsBioinformatics, distraction osteogenesis, gene expression, microarray, mesenchymal stem cells
Research GroupBiophysics and Bioengineering group
PublisherMDPI
JournalInternational Journal of Molecular Sciences
ISSN1661-6596
Electronic1422-0067
Publication dates
Online17 Jun 2021
Print17 Jun 2021
Publication process dates
Deposited21 Jun 2021
Accepted14 Jun 2021
Output statusPublished
Publisher's version
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File Access Level
Open
Copyright Statement

© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Additional information

This article belongs to the Special Issue Different Strategies for Osteogenic Differentiation and Bone Regeneration.

Digital Object Identifier (DOI)https://doi.org/10.3390/ijms22126505
LanguageEnglish
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