Stem cell engineered bone with calcium-phosphate coated porous titanium scaffold or silicon hydroxyapatite granules for revision total joint arthroplasty

Article

García-Gareta, E., Hua, J., Rayan, F. and Blunn, G. 2014. Stem cell engineered bone with calcium-phosphate coated porous titanium scaffold or silicon hydroxyapatite granules for revision total joint arthroplasty. Journal of Materials Science: Materials in Medicine. 25 (6), pp. 1553-1562. https://doi.org/10.1007/s10856-014-5170-z
TypeArticle
TitleStem cell engineered bone with calcium-phosphate coated porous titanium scaffold or silicon hydroxyapatite granules for revision total joint arthroplasty
AuthorsGarcía-Gareta, E., Hua, J., Rayan, F. and Blunn, G.
Abstract

Aseptic loosening in total joint replacements (TJRs) is mainly caused by osteolysis which leads to a reduction of the bone stock necessary for implant fixation in revision TJRs. Our aim was to develop bone tissue-engineered constructs based on scaffolds of clinical relevance in revision TJRs to reconstitute the bone stock at revision operations by using a perfusion bioreactor system (PBRS). The hypothesis was that a PBRS will enhance mesenchymal stem cells (MSCs) proliferation and osteogenic differentiation and will provide an even distribution of MSCs throughout the scaffolds when compared to static cultures. A PBRS was designed and implemented. Scaffolds, silicon substituted hydroxyapatite granules and calcium-phosphate coated porous TiAl6V4 cylinders, were seeded with MSCs and cultured either in static conditions or in the PBRS at 0.75 mL/min. Statistically significant increased cell proliferation and alkaline phosphatase activity was found in samples cultured in the PBRS. Histology revealed a more even cell distribution in the perfused constructs. SEM showed that cells arranged in sheets. Long cytoplasmic processes attached the cells to the scaffolds. We conclude that a novel tissue engineering approach to address the issue of poor bone stock at revision operations is feasible by using a PBRS.

PublisherSpringer US
JournalJournal of Materials Science: Materials in Medicine
ISSN0957-4530
Publication dates
Online12 Feb 2014
Print30 Jun 2014
Publication process dates
Deposited28 May 2015
Accepted30 Jan 2014
Output statusPublished
Digital Object Identifier (DOI)https://doi.org/10.1007/s10856-014-5170-z
PubMed ID24519756
LanguageEnglish
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